Isolation of a third isoenzyme of soybean lipoxygenase.
نویسندگان
چکیده
I. A third isoenzyme of soybean lipoxygenase (linoleate: oxygen oxidoreductase, EC 1.13.I.13) has been purified to essential homogeneity by Sephadex DEAEA5o column chromatography and isoelectric focusing. 2. The enzyme differs from the two previously described pure isoenzymes (lipoxygenases-i and -2) 1 by the following criteria: elution profile from DEAESephadex, isoelectric point, pH activity profile, and the effect of Ca 2+ on activity. 3. The new isoenzyme also shows an anomalous, inverse dependence of activity on enzyme concentration.
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ورودعنوان ژورنال:
- Biochimica et biophysica acta
دوره 284 1 شماره
صفحات -
تاریخ انتشار 1972